Isolation of Daudi Cells with Reduced Sensitivity to Interferon.: I. Characterization

Isolation of Daudi cells with reduced sensitivity to interferon. I. Characterization.

Treatment of Daudi cells with successively increasing concentrations of interferon-alpha resulted in the selection of a cell population which multiplied in the continued presence of 10(4) units/ml of interferon-alpha. A number of clones of interferon-resistant Daudi cells were isolated from this population. Two clones, DIF2 and DIF3, were found to exhibit moderate and pronounced resistance, res...

Isolation of Rickettsia prowazekii with reduced sensitivity to gamma interferon.

The growth of Rickettsia prowazekii Madrid E was monitored in mouse L929 cells subcultured for several weeks in the presence of gamma interferon (IFN-gamma) to determine whether the rickettsiae would be eliminated from or would persist in these cultures. R. prowazekii exhibited two distinct patterns in these IFN-gamma-treated cultures. In some cases, IFN-gamma-induced inhibition of rickettsial ...

Interferon-Resistant Daudi Cells

Reduced sensitivity of staphylococcus aureus to vancomycin

Introduction: Staphylococcus aureus is one of the most common causes of infections in hospitals and vancomycin is used for the treatment of methicillin resistant S, aureus infections. Therefore, imergence of Staphylococcus aureus with reduced sensitivity to vancomycin is a serious problem. The aim of this study was to investigate the prevalence of Staphylococcus aureus with reduced suscep...

Interferon - a Down - regulates Insulin Receptors in Lymphoblastoid ( Daudi ) Cells

The Daudi line of human lymphoblastoid cells requires insulin and transferrin for growth in serumfree medium and is highly sensitive to the inhibitory effect of human leukocyte interferon (IFN-a) on cell proliferation. A variant subline of Daudi cells, which is resistant to the antiproliferative action of IFN-a, also has been grown in serum-free medium containing insulin and transferrin. The pr...